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 | Volume 9, Number 1 (Spring 2007) |  | |
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 | Mitogenic Stimulation of Murine Lymphocyte Cells by Exposure to Staphylococcal Aureus Enterotoxin B | |
 | | Author(s): Imani fooladi A. A. *, Sattari M., Mohammad Hassan Z., Azizi T., Mahdavi M., Khoramabadi N., Sadrai S. H. and Hosainydoust S. R. | | Department of Microbiology, Faculty of Medicine, and Research Center of Molecular Biology, Baqiyatallah(a.s.) University of Medical Sciences imanifouladi.a@gmail.com | | Study Type: Research | Subject: military medcine | | | Article abstract: | Introduction. Exposure of T cells with staphylococcal enterotoxns invitro condition is known to activate T cells in a subset restricted manner based on β-chain variable region (Vβ) gene expression. In particular, staphylococcal enterotoxin B (SEB) activates T cells bearing V β8. Material and Methods. We examined the ability of SEB to activate T cells in vitro. Exposure of lymphocyte cells origin of lymph node Balb/c mice (auxiliary , Inguinal and Mesenteric node) with doses of SEB ranging from 1, 10, 100, 1000 and 10000 ng/µl resulted in a dose dependent activation of T cells as reflected by increased proliferation that was detected by MTT assay. Results. Our results showed that in order increase of proliferation T cells, 1000 ng/µl of SEB was the best concentration. In additional, we studied the ability of SEB in create of apoptosis with Hoechst staining. Our result demonstrate that in a statistically there are not significant difference between above SEB concentrations and negative control. Therefore 1000 ng/µl of SEB don’t create apoptosis but it is just inducible lymphocyte cell. Discussion. Staphylococcal Enterotoxins is the best super antigen, and induce T-Cell suppressor, that it increase tumor cell death. Therefore, SEB is a very excellent candidate for tumor therapy. | | | Keywords: Staphylococcal Enterotoxin B(SEB), Superantigen, Tumor, Lymphocyte cell, | |
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